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Description
Viral Nucleic Acid Isolation Kit (Silica-Based Spin Column) from Bioperfectus is specially designed for efficient purification of viral RNA and viral DNA from cell-free samples such as serum, plasma, urine, body fluids and the supernatant of viral infected cell cultures. The sample is lysed by incubation with Lysis Solution. The Lysis Solution inactivates RNases, ensuring protection of viral nucleic acids against degradation. The lysed sample is transferred to a spin column where released viral nucleic acids immediately bind to the silica-based filter in the presence of Binding Buffer. The remaining contaminants are removed during three wash steps using Wash Buffer, whereas pure nucleic acids remain bound to the membrane. Pure viral nucleic acids are released from the spin column filter using Elution Buffer. The purified nucleic acids are ready-for-use in subsequent downstream applications of molecular biology.